Journal: Viruses

Article Title: Characterization of Immune Response Diversity in Rodents Vaccinated with a Vesicular Stomatitis Virus Vectored COVID-19 Vaccine

doi: 10.3390/v14061127

Figure Lengend Snippet: Characterization of the recombinant VSV viruses. ( a ) Schematic diagrams showing genome organization of the rVSV. VSV g was replaced by SARS-CoV-2 S to generate rVSV-ΔG-S and rVSV-ΔG-S-eGFP. ( b ) Indirect immunofluorescence identification results of rVSV-ΔG-S and rVSV-ΔG-S-eGFP. Vero E6 cells were infected with rVSV-ΔG-S or rVSV-ΔG-S-eGFP and recognized by SARS-CoV-2-specific antibody and Alexa Fluor 568-conjugated secondary antibody, presented red fluorescence, rVSV-ΔG-S-eGFP presented green fluorescence. No fluorescence signal was observed in the VSV control. ( c ) Lysates of rVSV-ΔG-S/rVSV-ΔG-S-eGFP-infected Vero E6 cells, and VSV-infected Vero cells were blotted with an antibody recognizing SARS-CoV-2 S protein, a band representing S at 190 kDa was detected in rVSV-ΔG-S and rVSV-ΔG-S-eGFP group, no band was observed in the VSV control. ( d ) Growth kinetics of rVSV-ΔG-S, rVSV-ΔG-S-eGFP and VSV. Vero E6 cells were infected with the recombinant VSV viruses (MOI = 0.01) and virus titers in the supernatant were measured at the indicated time points post-infection.

Article Snippet: Rabbit polyclonal antibody against SARS-CoV-2 S protein (Cat. 40589-T62) was purchased from Sino Biological Inc (Beijing, China).

Techniques: Recombinant, Immunofluorescence, Infection, Fluorescence

Journal: Viruses

Article Title: Characterization of Immune Response Diversity in Rodents Vaccinated with a Vesicular Stomatitis Virus Vectored COVID-19 Vaccine

doi: 10.3390/v14061127

Figure Lengend Snippet: Virus-specific antibody and neutralizing antibody induced by rVSVΔG-S. ( a ) Weight change monitoring of BALB/c mice following vaccination for 7 days. i.m., i.n. and control refer to i.m. ( n = 10), i.n. inoculation group ( n = 10) and PBS inoculation control group ( n = 10), respectively same in ( b – f ). ( b ) RBD-specific IgG in mouse serum samples at 14, 28 and 42 dpv. ( c ) nAbs in the serum of mice at 14, 28, 42 and 56 dpv. ( d ) Weight changes in golden hamsters 7 days after vaccination. ( e ) RBD-specific IgG in the serum of golden hamsters at 14 and 28 dpv. ( f ) nAb titers in hamster serum samples at 14, 28 dpv and post-SARS-CoV-2 challenge. Data are presented as mean ± SEM. (* p < 0.05, ** p < 0.01,*** p < 0.001, **** p < 0.0001).

Article Snippet: Rabbit polyclonal antibody against SARS-CoV-2 S protein (Cat. 40589-T62) was purchased from Sino Biological Inc (Beijing, China).

Techniques:

Journal: Viruses

Article Title: Characterization of Immune Response Diversity in Rodents Vaccinated with a Vesicular Stomatitis Virus Vectored COVID-19 Vaccine

doi: 10.3390/v14061127

Figure Lengend Snippet: Antibody subtype and cytokines induced by rVSVΔG-S in BALB/c mice. I.m., i.n. and control refer to i.m. ( n = 4), i.n. inoculation group ( n = 4) and PBS inoculation control group ( n = 2), respectively (same in b–f). ( a ) RBD-specific IgG1 and IgG2a induced by the rVSV vaccine in mice. ( b , c ) Splenocyte IFN-γ and IL-4 induced by the rVSV vaccine in mice. ( d ) Ratio of IgG2a/IgG1 in i.m. and i.n. group. ( e , f ) CD3+CD4+ and CD3+CD8+ positive T cell proportions in splenocyte before and post-stimulation with 20 μg/mL RBD protein of SARS-CoV-2 for 36 h at 37 °C, 5% CO 2 . Data are presented as mean ± SEM. (* p < 0.05, ** p < 0.01, *** p < 0.001).

Article Snippet: Rabbit polyclonal antibody against SARS-CoV-2 S protein (Cat. 40589-T62) was purchased from Sino Biological Inc (Beijing, China).

Techniques:

Journal: Viruses

Article Title: Characterization of Immune Response Diversity in Rodents Vaccinated with a Vesicular Stomatitis Virus Vectored COVID-19 Vaccine

doi: 10.3390/v14061127

Figure Lengend Snippet: Protective efficacy of rVSV-ΔG-S in golden hamsters following SARS-CoV-2 challenge. i.m., i.n. and control refer to i.m. ( n = 5), i.n. inoculation group ( n = 5) and PBS inoculation group ( n = 2) who were challenged with SARS-CoV-2, respectively (same in b–f). Unchallenged control refers to hamsters inoculated with PBS and free from SARS-CoV-2 challenge ( n = 6). ( a ) Survival rate following SARS-CoV-2 challenge. ( b ) Body weight change in golden hamsters following SARS-CoV-2 challenge. ( c , d ) SARS-CoV-2 viral RNA copies in the lungs and nasal turbinates following SARS-CoV-2 challenge. ( e , f ) SARS-CoV-2 viral titers in the lungs and nasal turbinates following SARS-CoV-2 challenge. Data are presented as mean ± SEM. (* p < 0.05, *** p < 0.001, **** p < 0.0001).

Article Snippet: Rabbit polyclonal antibody against SARS-CoV-2 S protein (Cat. 40589-T62) was purchased from Sino Biological Inc (Beijing, China).

Techniques:

Journal: Viruses

Article Title: Characterization of Immune Response Diversity in Rodents Vaccinated with a Vesicular Stomatitis Virus Vectored COVID-19 Vaccine

doi: 10.3390/v14061127

Figure Lengend Snippet: Representative H&E staining and IHC of lung sections from SARS-CoV-2-infected golden hamsters at 3 days post-infection. Mock refers to hamsters inoculated with PBS and free from SARS-CoV-2 challenged. i.m., i.n. and control refer to i.m. inoculation group, i.n. inoculation group and PBS inoculation group who were challenged with SARS-CoV-2, respectively. In H&E staining sections, the thickened alveolar wall and proliferated epithelial cells are marked with yellow arrows, broken alveolar diaphragms and atrophic alveoli are marked with red arrows. In IHC sections, SARS-CoV-2 antigens that were recognized by SARS-CoV-2-N-specific antibodies were marked with red arrows.

Article Snippet: Rabbit polyclonal antibody against SARS-CoV-2 S protein (Cat. 40589-T62) was purchased from Sino Biological Inc (Beijing, China).

Techniques: Staining, Infection